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      Continuous Chromatography for Biotherapeutics

      A profound introduction to continuous chromatography for mAbs, Oligonucleotides, Peptides and Viral Vectors.

      Key data

      Next start
      9.-11.9.2025
      Duration
      3 Days
      Final application date
      31.7.2025
      Teaching language
      English
      Place
      FHNW Campus Muttenz
      Fee
      CHF 3’000

      8 - 12 participants

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      The aim of this seminar is to provide an introduction to continuous chromatography with hands-on practice with capture and polishing processes for biomolecules. These processes lead to improvements in productivity and manufacturing costs while reducing environmental footprint. Attendees will acquire the basic tools to design, run and evaluate multicolumn processes and to quantify these improvements, serving as basis for an economic evaluation. As the least complex of all multicolumn processes, the workshop is focused on twin column chromatography.

      • Introduction to continuous chromatography for biomolecules
      • Theory of multi-column chromatography
      • Design of multi-column chromatography processes
      • Hands-on training on twin column equipment for capture and polishing applications
      • Process performance evaluation and scale-up
      • Introduction to process modelling

      This workshop does not cover 4-zone SMB, chiral and small molecule separations.

      Content

      The seminar comprises presentations and interactive workshops using laboratory-scale Contichrom CUBE twin column separation & purification systems. Subject matter experts and graduate assistants will support the participants during the interactive workshops and data analysis sessions.

      • Production of Biotherapeutics
      • Fundamentals of Large-molecule chromatography
      • Intro to Continuous Chromatography (CaptureSMB)
      • Lab workshop: CaptureSMB
      • Modelling and Simulation
      • Digitalization
      • Evening program / Dinner

      Programme Day 1
      09.00-09.10: Welcome and Housekeeping
      09.10-09.45: Lecture 1 Production of Bio-Therapeutics
      09.45-10.45: Lecture 2 Fundamentals of Large-Molecule Chromatography
      10.45-11.00: Coffee break
      11.00-12.30: Lab Workshop 1 Contichrom CUBE and Batch Capture
      12.30-13.30: Lunch
      13.30-14.15: Lecture 3 Continuous Countercurrent Chromatography
      14.15-15.00: Lecture 4: Affinity (Capture) Chromatography
      15.00-15.15: Coffee break
      15.15-17.00: Lab Workshop 2 CaptureSMB
      17.00-18.00: Lecture 5: Performance Evaluation of Continuous Chromatography
      18.00: Reception

      • Flowthrough and Frontal Chromatography
      • Integrated Chromatography
      • Continuous Chromatography: MCSGP
      • Lab workshop: MCSGP
      • Performance Evaluation
      • Lab workshop: CaptureSMB Evaluation
      • N-Rich
      • Evening program / Dinner

      Programme Day 2
      09.00-10.30: Lecture 6 Bind and Elute (Polishing) Chromatography
      10.30-10.54: Coffee break
      10.45-12.00: Lab Workshop 3 Linear Gradient Chromatography
      12.00-13.00: Lunch
      13.00-13.30: Lab Workshop 4 Evaluation Center
      13.30-14.30: Lab Workshop 5 Evaluation of Batch and CaptureSMB
      14.30-15.00: Presentation of Capture Results by Groups
      15.00-15.15: Coffee break
      15.15-17.30: Lab Workshop 6 MCSGP
      17.30: Social programme + Dinner

      • Modelling Workshop
      • Lab workshop: MCSGP Evaluation
      • Scale-up (MCSGP)
      • Wrap-up

      Programme Day 3
      09.00-10.30: Lecture 7 Integrated Continuous Manufacturing & Process Digitalization
      10.30-10.45: Coffee break
      10.45-12.00: Lecture 8 Modeling and Simulations
      12.00-13.00: Lunch
      13.00-14.00: Lab Workshop 7 Evaluation of MCSGP
      14.00-14.30: Presentation of Polishing Results by Groups
      14.30-15.15: Lecture 9-I Scale-up of continuous chromatography (part I)
      15.15-15.30: Coffee break
      15.30-16.15: Lecture 9-II Scale-up of continuous chromatography (part II), Guest Speaker: Ralf Eisenhuth, Bachem, Switzerland
      16.15-16.45: Lecture 10 N-Rich Process for Isolation of Impurities
      16.45-17.00: Course Wrap-up
      17.00-17.30:Guided lab tour (optional)

      Overview

      This seminar is aimed at industry and academic separation scientists and process development engineers who already have some familiarity with single column chromatography and who want to broaden their understanding of chromatographic processes and look at new and more efficient ways to separate and polish biomolecules.

      The seminar comprises presentations and interactive workshops using laboratory-scale Contichrom CUBE twin column separation & purification systems. Subject matter experts and graduate assistants will support the participants during the interactive workshops and data analysis sessions.

      The seminar fee is CHF 3’000.
      This includes lecture summaries in paper and electronic formats, materials used during the workshop, internet access (Wifi), lunch and coffee breaks as well as participation in the evening pro-gram/dinner. It does not include accommodation, travel costs or catering other than indicated above.

      Massimo Morbidelli Ph.D., Professor of Chemical Reaction and Separation Technologies in the Department of Chemistry, Politecnico di Milano. A pioneer in preparative continuous chromatography and in particular in the application of multicolumn technologies for protein purification in the pharma industry, Prof. Morbidelli has co-authored over 500 research articles and four books. He serves as associate editor for the Industrial & Engineering Chemical Research journal of the ACS and is the recipient of the 2005
      RH Wilhelm award from the AIChE and of the 2014 Gerhard Damkoehler medal of DECHEMA. He is a co-founder of ChromaCon AG in Zurich.

      Thomas Müller-Späth, Ph.D., Director of RnD at ChromaCon AG in Zurich. After an assignment at Bayer Health-care, Thomas completed his doctoral work on continuous chromatography of biomolecules in the group of Prof. Morbidelli, and co-founded ChromaCon AG to bring the technology to the market. He has been working on research projects with industrial partners, development of chromatography processes and equipment, and IP management. He has presented on numerous workshops and conferences on continuous chromatography and has co-authored over 20 publications and patents.

      Supervisors and tutors: Sebastian Vogg, Richard Weldon, Lars Aumann

      Testimonials

      “The continuous chromatography seminar had an excellent balance of theoretical content and laboratory based exercises. It was great to explore the significant gains observed in productivity, buffer consumption and resin utilization over batch chromatography.”

      “Great to learn all the potential and application of the different ways of using continuous chromatography and learn that this is not just a smart way of working in research, but that it can be implemented in large scale production.”

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      FHNW Campus Muttenz

      FHNW University of Applied Sciences and Arts Northwestern Switzerland

      Hofackerstrasse 30

      4132 Muttenz

      Phone+41 61 228 55 55

      E-Mailempfang.muttenz@fhnw.ch

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