The combination of membrane-supported liquid-liquid extraction with inline concentration measurement of the two phases allows the mass transfer to be monitored continuously without additional offline analysis. Since mixing and separation of the two liquid phases is not required, the process can be operated continuously without long settling times. In discontinuous…
Building accurate and robust Raman models for online monitoring of cell culture processes remains a difficult and time-consuming task. A novel offline calibration approach based on design-of-experiment spiking and multivariate data analysis was developed to monitor of several cell culture components simultaneously. Raman prediction models enabled real-time insight into the…