Development of a new Coupling Strategy for Enzyme-conjugated Antibodies

For ELISAs and Western Blot antibodies are labelled with fluorophores or with enzymes. For the conjugation with enzymes a classic but rather unspecific coupling with glutaraldehyde is commonly used. The reaction with glutaraldehyde leads to the formation of large aggregates of antibodies and enzymes with varying quality. In this project we used recombinantly expressed bacterial Alkaline Phosphatase and coupled it to antibodies with a highly specific heterobifunctional crosslinker which allowed the preparation of defined antibody-AP conjugates with high enzymatic activity.

Funded by Innosuisse.